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Faculty Profile Last Modified Time: 11:56:45 AM Fri, 27 Mar 2020   Contact Information Eric Wagner Associated Profiles  Associate Professor, Biochemistry & Molecular Biology, UTMB   Mail Box: 0645, Basic Science Building, Room No.: 5.104B    ejwagner@utmb.edu     (409) 772-0027    Web Link    Lab Webpage    Polypyrimidine Tract-Binding Protein, Small Nuclear RNA, Histones, Messenger RNA, Exons, RNA Interference, Polyadenylation, Alternative Splicing    Associated Profiles Professional Preparation Research and Expertise Affiliations Appointments  Professional Preparation  Degree Major Institution Year  PhD Molecular Cancer Biology Duke University 2002  BS Molecular Genetics University of Rochester 1997  Research and Expertise Research Interests   Project 1: Alternative Polyadenylation in Cancer. In non-proliferating or non-transformed cells, the position of cleavage and polyadenylation is located as distal as possible relative to the stop codon. The use of this distal poly(A) site (dPAS) ensures maximal negative regulation by microRNAs as well as AU-rich destabilizing elements. The translational output of these longer mRNAs is therefore under more exquisite control by the cell. However, during the processes of transformation, tumor progression, or enhanced proliferation; the decision of where to put the poly(A) tail is altered and a more proximal poly(A) site (pPAS) is chosen. This typically does not change sequences within the open reading frame, the reduced 3'UTR length removes negative regulatory elements increasing protein expression. In the Wagner laboratory, we are focused on the mechanism of this change and the critical target mRNAs that undergo this process. We use a combination of cellular and in vivo models to investigate APA as it relates to tumor biology. Project 2: The Role of the Integrator Complex in Gene Expression Regulation. The Integrator Complex was discovered in 2005 as a 12 membered group of proteins that are required for the 3' end formation of small nuclear RNA (snRNA). The Integrator Complex is thought to interact with machinery at the snRNA promoter including members of the SNAP complex, which leads Integrator association with the CTD of RNAPII. The molecular details of these interactions have not been elucidated to this date. Once Integrator is loaded onto RNAPII, it will cleave the pre-snRNA using its catalytic RNA endonuclease subunit: IntS11. More recently, Integrator has been shown to function in the pause/release of RNAPII at immediate early genes. It plays a key role in the recruitment of pTEF-b and ultimate transcriptional activation through enhanced elongation competence. The Wagner laboratory is interested in determining the mechanism of Integrator action at these various gene types. To that end, we have developed model systems in both human and Drosophila cells in address its various unknowns.  Publications/Creative Works Click here to search for this faculty member's publications on PubMed.  Affiliations  Training Program Computational Cancer Biology  Appointments Title Department / School Institution Associate Professor Biochemistry and Molecular Biology UT Medical Branch at Galveston

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