It is believed that neuronal activities in the individual neuronal network module, such as barrel and column, build up the fundamental function of the network module and the mutual interaction among the network modules are reflected in the brain function. To understand the brain mechanism at the neuronal network level, it is important to clarify how individual neuronal activities are processed in the network module. Despite much study, the functional basis of the modules has remained unclear because it is difficult to detect individual neuronal activities in the context of their neuronal networks. Applying the in vivo electroporation neuronal labeling method to the two-photon functional imaging, I am currently investigating the questions using the olfactory glomerular module as a model system. This will in turn, allow us to understand not only the basic function the glomerular module plays in odor coding and smell perception but also the general functional rule of the neuronal network modules.
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