Our lab is broadly interested in developing and applying genomic technologies to understand the genetic networking underlying developmental biology and human diseases. Both experimental and computational approaches are used in combination to identify and model gene functions in both human patients and model organisms, including Drosophila and mice. Identification of human retinal disease genes: One of the main focuses in our laboratory is to understand the molecular mechanism underlying human retinal disease. Collectively, ocular diseases affect a large population; approximately 40 million people in the world are blind and another 100 million have substantial visual impairment. Together with our collaborators, we are currently working on identifying disease genes involved in several human retinal diseases, including Leber congenital amaurosis (LCA), Usher syndrome, retinitis pigmentosa (RP), and AMD. Recently, we have cloned SPATA7 as a novel human retinal disease gene, mutations in which lead to LCA and RP. In addition, several novel disease loci have been mapped in our patient collection. To identify the mutations in these loci, we are applying the cutting edge NextGen sequencing technologies to perform both targeted and whole genome sequencing on these patient DNA samples. Animal models for retinal disease and development: Model organisms including mouse and Drosophila melanogaster are useful tools to understand molecular mechanism of diseases and also identify genetic networks that control retinal development. Using mouse as the model organism, we have recently generated numerous knock out mice that mimic human retinal diseases. Genetic, genomic, and biochemical approaches to decipher the molecular function of these genes are currently underway. In Drosophila, a major effort in our laboratory is to understand the molecular mechanism of the early retinal cell fate determination process. A genome-wide, combinatorial approach, including gene expression profiling with both microarray and mRNA-Seq, comparative genomics at both DNA and mRNA level, and downstream target identification using ChIP-Seq, has been adopted. Strikingly, our data suggests a highly connected, dynamic genetic network. Further characterization as well as experimental validation and testing of the network will likely to provide a significant contribution to our understanding of the genetic mechanisms controlling retinal development in general. Genomic technology development and applications: Introduction of new technologies often leads to breakthrough of scientific discoveries. Recently, the most exciting novel technology in molecular and genomic biology is the Next generation sequencing. To fully utilize this in our research, a set of protocols and software tools that is specific for the NextGen technology has been developed among our laboratory and our collaborators, including RNA-Seq, miRNA-Seq, CNV-Seq, ChIP-Seq, chromatin profiling, and mutation detection. Currently, we are applying these tools to various research fields, including development, genetic disease gene cloning, and cancer biology.
Publications/Creative Works
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Affiliations
Training Grants
Training in Precision Environmental Health Sciences (TPEHS)
NLM Training Program in Biomedical Informatics & Data Science for Predoctoral and Postdoctoral Fello
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